host strain meaning in English
寄助株
Examples
- Two main parts were included in the present thesis . part i . cloning and expression cryigenes of bacillus thuringiensis in different host strains
一、苏云金芽胞杆菌杀虫晶体蛋白基因在不同受体菌中的表达1 - It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation
通过电转化作用该基因片段被成功地整合至酵母cs115的染色体上，经过甲醇诱导之后，该基因得到了分泌表达。 - Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol
我们提取了km71ppic9k - fl转化菌株的基因组dna进行southern实验，检测目的基因的整和插入；提取总rna ，进行了northern实验，检测fl基因在转化菌株中的表达。 - A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e . coli jm109 as the host strain . two recombinants , pgr3 and pgr7 , which can confer glyphosate resistance ofe . coli jm109 were identified from the selective medium containing 10mm glyphosate
以粘粒pla2917为载体、大肠杆菌jm109为受体菌构建荧光假单胞菌g2的基因组文库，在含有10mm草甘膦的固体选择培养基上筛选出两个耐受克隆pgr3和pgr7 ，插入片段分别为7kb和11kb 。 - The recombination vectors were transformed into host strain of bl21 and induced with iptg . all the recombinant protein was expressed into inclusion body . the recombinant protein was identified with sds - page and westen - blot and purified through elution method . the muticlone antibody was got by immuning the rabbit with the purified protein
把重组质粒转化入表达受体菌bl21 ，经iptg诱导后都获得了表达，且都以包涵体的表达形式存在，用sds - page和western - blot的方法对重组蛋白进行了鉴定。